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The present work describe simple, sensitive, rapid, accurate, precise and economical first derivative spectrophotometric (zero crossing) method for the simultaneous determination of Terbutaline and Etophylline in fixed dose combination.The first order derivative absorption at 280 nm (zero cross point for Etophylline) and 272.5 nm (zero cross point for Terbutaline) was used for Terbutaline and Etophylline determination, respectively. The linearity of Terbutalineand Etophyllinewas observed in the range of 5-30 μg/ml with correlation coefficient (r2) of 0.9981 and 0.9979, respectively.The mean % recoveries were found to be in the range of 100.09 % and 96.86 % for Terbutaline and Etophylline, respectively. The method was validated as per ICH Q2(R1) guidelines.

DEVELOPMENT AND VALIDATION OF FIRST ORDER DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF TERBUTALINE AND ETOPHYLLINE