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Leukotrienes (LTs) producing capacity was investigated in calcium ionophore A23 187- stimulated rabbit. rat and mice peripheral white blood cells suspension. A reverse phase high performance liquid chromatography technique and computerized UV spectroscopy were employed to isolate and quantitate the released LTs namely. LTC4 and LTB4. Preincubation of rabbit white blood cells at 37°C for 5 min followed by calcium ionophore-A23 187 (1 pM) stimulation for another 5 min produced an equal amounts of LTC4 as compared to LTC4 produced by human white blood cells (105±11 versus 95±9.5 pmol/107 cells respectively; mean ±SEM). In contrast rabbit white blood cells synthesized significantly lower LTB4 in comparison with LTB4 produced by peripheral white blood cells from healthy control (168±18 versus 228±19 pmol/107 cells respectively: mean ±SEM). When rat and mice white blood cells suspension were stimulated with calcium ionophore A23187 (1 µM) after preincubation at 37°C for 5 min, equivalent amounts of LTC4 and LTB4 were observed. However, LTB4 and LTC4 produced by rat and mice white blood cells were significantly lower in comparison with LTB4 and LTCj produced by human white blood cells stimulated with calcium ionophore-A23 187. These results demonstrate that rabbit. rat and mice white blood cells suspension possess the capacity to produce LTC4 and LTB4 from endogenous substrate after calcium ionophore-A23 187 stimulation.

Formation of Leukotrienes From Calcium Ionophore-A23187 Stimulated Rabbit, Rat and Mice White Blood Cells