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The maximum conversion parameters of sucrose and fructooligosaccharides (FOS) from a clarified yacon extract (1% FOS) to fructose were optimized by using chemical and enzymatic hydrolysis. Chemical hydrolysis was performed using sulfuric and phosphoric acids and enzymatic hydrolysis with inulinase and invertase enzymes. Surface methodology response a Instituto de Biotecnología (IBT), área de Biotecnología Industrial y Bioprocesos. Universidad Nacional Agraria La Molina (UNALM). Av. La Molina s/n, La Molina. Lima – Perú. (*) autor de correspondencia: dcampos@ lamolina.edu.pe Hidrólisis química y enzimática de extracto de yacón (Smallanthus sonchifolius) para la producción de... 201 Rev Soc Quím Perú. 83(2) 2017 was employed for optimization hydrolysis. A Box-Behnken design, was used for chemical hydrolysis, where optimal conditions using sulfuric acid were: pH 1, temperature 88,4°C and time of 38,7 min reaching 9,11 g fructose/L extract while with phosphoric acids were: pH 1, temperature 89,9°C and time of 42,2 min obtaining 7,84 g fructose/L extract. To enzymatic hydrolysis a central compose design was used, founding as optimal conditions with inulinase a time of 134 min and a enzymatic concentration of 11,7 U/g FOS and with invertase a time of 127,5 min and a enzymatic concentration of 6,74 x 10-1 U/g FOS (both reactions under constant conditions of temperature 50°C and pH of 5) reaching as maximum 6,16 and 7,50 g fructose/L extract, respectively. The obtained response surface models produced a satisfactory fitting of the results for both hydrolysis methods (R2 entre 0,880 y 0,999).

HIDRÓLISIS QUÍMICA Y ENZIMÁTICA DE EXTRACTO DE YACÓN (Smallanthus sonchifolius) PARA LA PRODUCCIÓN DE FRUCTOSA