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The effect of storage time of cloud ear fungus (Auricularia polytricha) spent culture media (SCM) made of three kinds of tropical hardwood species (Falcataria moluccana, Tectona grandis, and Shorea sp.) was investigated on their saccharification efficiency as a potential pretreatment of reducing sugar production. When SCM was stored at 25°C for 2 months (SCM-2), the chemical component analysis showed that lignin content was decreased (26.3 49.4%) by storage process compared with unstored SCM. The glucose yield by enzymatic saccharification increased from 7.3 9.0% to 10.1 12.8%. The highest reducing sugar yield (189.2 mg/g dry spent medium) with the highest hydrolysis weight decrease (24.2%) was obtained in the SCM-2 from Shorea sp. Therefore, the optimal storage time is different for each SCM, and storage of SCM is a very useful pretreatment for reducing sugar production.

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The Effect of Storage Time of Cloud Ear Fungus (Auricularia polytricha) Spent Culture Media Made of Three Indonesian Tree Species on Their Saccharification Rate