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We present double-tracer autoradiography with 35S and 32P using imaging plates and an aluminum plate to visualize the individual distribution of sulfur and phosphorus in Brassica napus L.. Because of the different attenuation efficiencies of β-rays emitted from 35S and 32P, the specific image of 32P was taken when an aluminum plate was placed between the sample and the imaging plate. The specific image of 35S was obtained by subtracting the 32P image from the 35S and 32P image. It was observed that high amount of 35S was distributed at the edge of the leaves and in root tips. The 32P was distributed throughout the whole plant and it was found that the phosphorous amount was higher at the shoot apical meristems than those in leaves.

Double-Tracer Radiography with Sulfur-35 and Phosphorus-32 Using Imaging Plates in Brassica napus L.