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In order to assess the role of age, prior test experience and prenatal maternal stress on open-field behavior, both stressed (treated) and control litters were divided into two groups. Group 1 was tested in the open field at both 40 and 120 days of age, and Group 2 at only 120 days. Comparison of activity level and location of activity in the open field of naive Ss at 40 days of age with those of naive Ss at 120 days of age demonstrated significant age effects. Similarly, comparisons of these behaviors of experienced Ss at 120 days with those of naive Ss at the same age, indicated effects of prior test experience. In addition, possible interactions of both age and prior test experience with the effects of prenatal stress were indicated. The importance of these findings for the design of studies in which the effects of experimental variables are examined at several different ages is discussed.

Open field behavior of C57BL/6J mice as a function of age, experience, and prenatal maternal stress