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Goldfish (Carassius auratus) were run for 4 days at 20 trials a day with either CS onset or CS offset conditions. In each condition, the CS was located on the same, the opposite, or both ends as the subject. Conditioned avoidance shuttle rate (CASR) was found to be a function of the magnitude of CS change for both CS onset and CS offset conditions, specifically from maximum to minimum CASR performance, both, same, opposite. The differences in this order of CASR performance vs. that where subjects are trained in a single day are interpreted as being a function of a negative phototaxis for the CS onset and a confusion reaction for the CS offset conditions, both of which affect acquisition rates in the single-day paradigm, but which acquire cue functions and augment acquisition in the multiday paradigm.

Spatially located visual CS effects on conditioned avoidance shuttle response (CASR) acquisition in goldfish (Carassius auratus): Training over days