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Galaxea, Journal of Coral Reef Studies 13: 29-34(2011) Abstract Quantifi cation of zooxanthellae densities in tissues of reef-building corals aids in the assessment of the extent and severity of coral bleaching. Various meth- ods are available to quantify zooxanthellae densities; how ever, a direct comparison of these techniques has yet to been done. Here, we compare estimates of zooxanthellae densities obtained using conventional airbrushing coupled with post-tissue-blasting surface area determination, ver- sus a technique whereby zooxanthellae densities are quantifi ed from a known area (0.25 cm 2 ) of tissue after corals have been fi xed and decalcifi ed. Estimates of zoo- xanthellae densities obtained were correlated across re- plicate colonies (R 2 =0.40, n=81), and both techniques revealed similar patterns of variation among locations. The main benefi t of the decalcifi cation technique was reduced processing time, because the technique eliminates the time-consuming process of tissue blasting and re- trospective estimates of surface area. We estimate that decalcifi cation halves the processing time per sample, and produces a more accurate estimate of zooxanthellae density.

A comparison of two methods of obtaining densities of zooxanthellae in Acropora millepora