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104 DOI: 10.37398/JSR.2020.640213 Abstract: Prognostic tool or a test of cure to monitor drugs response is not available for leishmaniasis till date. Individuals suffering with visceral leishmaniasis (VL) show seropositivity to all currently used diagnostic antigens even after years of successful cure. In this study, we explored a 13kDa excretory-secretory component of L. donovani, which showed seronegativity with serum obtained from patients on completion of treatment. Leishmania donovani promastigotes were grown in Dulbecco’s modified eagle media. The leishmanial excretory-secretory antigens (LESAs) were prepared and recovered from parasites free culture supernatant. The diagnostic and prognostic applicability of 13kDa protein was evaluated by enzyme linked immunosorbent assay. Result indicates that patient’s sera bears seropositivity for 13kDa protein before treatment and found to be antibodies negative after completion of treatment. The sera samples of various controls did not show seropositivity with this protein. The ELISA sensitivity of this protein was observed to be 100% at absorbance cut-offs 0.180 and 0.360. The specificity at absorbance cut-off 0.180 in nonendemic, endemic and disease control groups was observed to be 98%, 92% and 94%, respectively. The homology modeling predicted its homology with ubiquitin like protein. The identified protein was found to be highly sensitive and specific for serum antibodies present in VL patients. The findings also indicated its prognostic potential, which can also be exploited to monitor drug responses.

Diagnostic and Prognostic Applicability of an Ubiquitin like 13kDa Leishmania donovani Excretory-Secretory Protein for Visceral Leishmaniasis