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The degree of citrulline interference in the spectrophotometry of urea with diacetyl monoxime was determined, and the color products of the method were separated with high-performance liquid chromatography. From the peak heights of the color products of urea and citrulline with diacetyl monoxime, the citrulline interference can be moderately high, but, in most cases it is insignificant. In 59 out of 70 water samples from eutrophic Lake Suwa in Japan, the concentration of citrulline was under the determination limit (<0.01 ƒÊg -at-N •E 1-1 urea equivalent). In the rest, citrulline contributed an average 8% (range 5-19%) to the color developed.

Evaluation of the degree of citrulline interference in the spectrophotometry of urea with diacetyl monoxime in natural waters.