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The present paper describe the production and characterization of novel collagen films containing propolis encapsulated in chitosan nanoparticles, for biomedical applications such as cutaneous wound healing. Structural and morphological details were investigated by ATR FTIR spectroscopy, SEM and nanoindentation measurements, revealing the collagen fibers aligned in a quasi-parallel distribution, which might be favorable for biomedical applications. Moreover, the vibrational marker bands of propolis were well preserved in the final polymeric mixture, indicating the stability of bioactive compounds upon the encapsulation procedure. The antibacterial effect depends on the nanoparticles concentration in collagen film, the effect being more evident with respect to E. coli than S. aureus. The antioxidant capacity monitored by CUPRAC assay, indicated a synergic effect of chitosan nanoparticles matrix and propolis extract, incorporated in collagen films.

Natural Polymeric Film Encapsulating Propolis Nano-Formulation for Cutaneous Wound Healing