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This paper reports the chemical constituents and antimicrobial activities of essential oil of the leaf and pseudo-stem of Zingiber zerumbet (L.) Smith. The air-dry samples were subjected to hydrodistillation process using Clevenger-apparatus to obtained essential oils. By using GC and GC/MS, 55 and 25 compounds representing 96.2% and 94.4% of the total oil contents were determined from essential oils of the leaves and pseudo-stem respectively. The main compounds of the leaf oil were -pinene (30.8%), -caryophylene (13.0%) and caryophyllene oxide (12.0%) while zerumbone (64.5%) was the most abundant constituents found in the pseudo-stem oil. The antimicrobial activity (MIC) was determined by microdilution broth susceptibility assay. The results of antimicrobial testing indicated that Z. zerumbet leaf oil exhibited potent antimicrobial action against Aspergillus niger (ATCC 9763) with minimum inhibitory concentration (MIC) of 25 μg/mL. The pseudo-stem oil only displayed antimicrobial activity towards B. subtilis (ATCC 11774) with MIC of 50.0 μg/mL.

Antimicrobial Activity and Constituents of Leaf and Pseudo-Stem Essential Oils of Zingiber zerumbet