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Disease is a major factor affecting maize production. The utilization of resistant cultivars is the most economical, safe, and effective method for controlling maize diseases. This research was finally conducted using 2000 maize germplasm accessions with extensive sources and rich genetic background to identify and evaluate the resistance to multiple diseases in multi-plot demonstration for several years under natural condition during 2016–2019. Nine diseases, including southern corn leaf blight, stalk rot, common smut, Curvularia leaf spot, southern rust, maize rough dwarf, northern corn leaf blight, gray leaf spot and head smut were investigated and evaluated in non-inoculated fields. The experimental data at 10 different sites from 2016 to 2019 showed that resistance identification under natural conditions was sensitive to environmental factors, with distinct differences among years and regions. The incidence of southern corn leaf blight was severe at multiple sites over these years and 11 maize accessions with

A large-scale screening of maize germplasm for resistance to multiple diseases in multi-plot demonstration for several years under natural condition