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This experiment amid to study the effect of cultivars, growth regulator and number of sub-cultures on the shoots formation of strawberry plantlets during multiplication stage and Random amplified polymorphic DNA (RAPD) analysis of strawberry cultures in vitro during the fourth sub-culture. This experiment included 40 treatments, which were the combination between two strawberry cultivars (Festival and Sweet Charlie), five treatments of growth regulator (BA and GA3) and four number of sub-cultures during shoots formation (multiplication stage). The obtained results showed that, the maximum increment of growth measurement of strawberry plantlets were recorded by Sweet Charlie cultivar .In addition , using 1⁄2 MS-medium without supplemented with any growth regulators (BA and GA3) being the superior treatment for increasing both number of leaves per shoot and shoot length. On the other hand, generally, the fourth sub-culture being the most effective treatment on the growth measurement of strawberry plantlets during multiplication stage. Furthermore, Random amplified polymorphic DNA (RAPD) analysis varied according to the two tested cultivars and the type of for production of disease resistant plants and in plant breeding and crop improvement programs (Mohamed, 2003).

EFFECT OF CULTIVARS, GROWTH REGULATOR AND SUB-CULTURE ON THE PLANTLET SHOOTS FORMATION AND RAPD ANALYSIS OF STRAWBERRY CULTURES IN VITRO