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The aim of the study was to evaluate the diagnostic parameters of various methods for determining allergy sensitization to the dust mite allergen Dermatophagoides pteronyssinus in patients with respiratory allergic diseases – allergic rhinitis and bronchial asthma.Materials and methods: During the study, 88 patients with allergic rhinitis and / or atopic asthma were examined with three different methods of specific allergic diagnosis (in vivo and in vitro) according to the recommendations of the ethics committee of the Pirogov Vinnitsa National Medical University, all of which were out of acute period. Inclusion criteria were a diagnosis of allergic rhinitis (both intermittent and persistent) and / or atopic asthma. The prick-test was conducted according to the classic test method by normative documents with commercial allergen extracts. Western blots for the determination of IgE levels were performed using RIDA AllergyScreen test systems (R-Biopharm AG, Darmstadt, Germany) and Euroline (Euroimmun).Results and discussion: The results of two systems for the determination of specific IgE to D. Pteronyssinus by the Rida AllergyScreen and Euroline methods have a systematic difference of values (-1.27 kU / l).There is a good agreement between the results of skin testing with D. Pteronyssinus allergens and the detection of specific IgE by the Rida AllergyScreen method, and there is a satisfactory agreement between the results of skin testing with D. Pteronyssinus allergens and detection of specific IgE by the Euroline method.According to the results of the curve of detection of specific IgE by AllergyScreen method for determination of sensitization to allergen D. Pteronyssinus has excellent diagnostic significance (AUC=0,953), detection of specific IgE by Euroline method for determination of sensitization to allergen D. Pteronyssinus – good AUC=0,7.

etkinset 12.sınıf etkin çalışma dergisi lys coğrafya

Comparison of diagnostic parameters of methods for determination of sensitization to allergen D. pteronyssinus in patients with respiratory allergic diseases