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46 Introduction  47 5 Experimental section  49 2.3.1 Materials  49 2.3.2 NOC-7 treatment and fluorescence imaging  49 2.3.3 LPS treatment and fluorescence imaging  50 Results and Discussion  50 2.4.1 Synthetic approach to fluorescent probe for NO  50 2.4.2 Optical properties of BODIPY dyes  51 2.4.3 The fluorescent response and selectivity of the fluorescent probe A to NO  53 2.4.4 Use of fluorescent probe to detect nitric oxide in living cells  56 Conclusion  60 Abstract  68 Introduction  68 Experimental Section  70 3.3.1 Instrumentation.  70 3.3.2 Materials  71 3.3.3 Cell culture and confocal fluorescence imaging  74 Results and Discussion  75 3.4.1 Design and synthesis of near-infrared fluorescent probes for pH  75 3.4.2 Optical responses of fluorescent probes to pH  76 3.4.3 The selectivity experiments of fluorescent probes to pH over metal ions.  80 3.4.4 Live cell imaging of fluorescent probes  81 6 68 Introduction  68 Experimental Section  70 3.3.1 Instrumentation.  70 3.3.2 Materials  71 3.3.3 Cell culture and confocal fluorescence imaging  74 Results and Discussion  75 3.4.1 Design and synthesis of near-infrared fluorescent probes for pH  75 3.4.2 Optical responses of fluorescent probes to pH  76 3.4.3 The selectivity experiments of fluorescent probes to pH over metal ions.  80 3.4.4 Live cell imaging of fluorescent probes  81 6

DESIGN, SYNTHESIS AND APPLICATIONS OF FLUORESCENT AND ELECTROCHEMICAL PROBES