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This thesis, "Optimization of ethanol production by yeasts from lignocellulosic feedstocks," is hereby approved in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE in BIOLOGICAL SCIENCES. iii Acknowledgements I would like to take this opportunity to express my extreme gratitude and appreciation to the people who have generously contributed to my success as a graduate student. I will forever be indebted to Dr. Susan Bagley who graciously and patiently served as my advisor. Dr. Bagley provided me with excellent academic and professional opportunities as well as guidance and support throughout my time at MTU. She is directly responsible of me being able to achieve the things I have as a graduate student. I greatly appreciate the advice and support of my Graduate Committee. Dr. David Shonnard and Dr. Rupali Datta brought an interdisciplinary approach to my education. Their broad range of knowledge and backgrounds of fields outside of microbiology only strengthen my education. Wisconsin for so generously providing MTU with Pichia stipitis CBS 6054. I would like to thank General Motors for so generously funding the project. Morinelly for their technical support and guidance. iv Finally, I would like to thank my family and friends that who were always there for me regardless of the situation or need. My deepest thanks go out to my parents, Rick and Cindy Gleason, and husband, Nathan Groves, for their never ending encouragement and guidance. I would also like to mention that I could not have done this without the help from my best friends: Thanks to all my MTU friends that made my time here one I will never forget.