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espanolEn la investigacion se describe morfologicamente amebas del genero Arcella sp mediante el uso de tecnicas de cultivo, microscopia electronica de barrido (MEB), y morfometria. Para ello, se tomo una muestra de 5μl de estos microorganismos en un medio de cultivo salino, estandarizado previamente. Al tratarse de una muestra acuosa, se realizo una estandarizacion del metodo utilizado para observacion a traves del MEB utilizando una modalidad de bajo vacio o fuerza ambiental. En este sentido, las muestras fueron fijada con 5μl de solucion Karnovsky al 2% por 15 min y post-fijada con 5μl de solucion de tetraoxido de osmio al 1% por 15 min. Posteriormente, se tomo una muestra de la mezcla, que se coloco en una rejilla de cobre cubierta previamente con una pelicula de nitrocelulosa y una pelicula de carbono para mayor resistencia mecanica de la rejilla, la cual se dejo secar en una estufa a temperatura ambiente por 20 min, una vez colocada la muestra. Finalmente, se observo en un microscopio electronico de barrido en modalidad de bajo vacio (ESEM) y a partir de las imagenes digitalizadas, se obtuvieron las dimensiones de las caracteristicas diagnosticas de interes, con la ayuda del programa ImageTool 3.0. Los resultados obtenidos de este estudio permitieron describir de forma general las caracteristicas diagnosticas de un grupo de amebas testadas pertenecientes al genero Arcella sp. EnglishIn the research morphologically, amoebas of the genus Arcella sp are described by the use of culture techniques, scanning electron microscopy (SEM), and morphometry. For this, a sample of 5μl of these microorganisms was taken in a saline culture medium, previously standardized. Being an aqueous sample, a standardization of the method used for observation through the MEB using a low vacuum mode or environmental force. In this regard, the samples were fixed with 5μl of Karnovsky solution at 2% for 15 min and post-fixed with 5μl of 1% osmium tetraoxide solution for 15 min. Subsequently, a sample of the mixture was taken, which was placed in a copper grid previously covered with a nitrocellulose film and coated with a carbon film for greater mechanical strength and allowed to dry in an oven at room temperature for 20 min, once the sample was placed. Finally, it was observed in a scanning electron microscope in low vacuum mode (ESEM) and from the digitized images, the dimensions of the diagnostic characteristics of interest were obtained, with the help of the ImageTool 3.0 program. The results obtained from this study allowed to describe in a general way the diagnostic characteristics of a group of tested amoebas belonging to the genus Arcella sp.

Descripción morfológica de amebas testadas del género Arcella sp., a través del uso de técnicas multiples