English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background: Localized methods of drug delivery, such as controlled drug release from fibrin glue (FG), has the potential of improving the efficacy of chemotherapy for brain tumors by bypassing the blood brain barrier. Photochemical internalization (PCI) of bleomycin (BLM) and doxorubicin (DOX) has been shown to enhance the cytosolic delivery of drugs in localized site and temporal specific manner. This study examined the ability of PCI to enhance the growth inhibiting effects of BLM or DOX released from FG on multi-cell glioma spheroids in vitro. Materials and Methods: FG layers consisted of a 1:1 ratio of fibrinogen and drug or photosensitizer loaded thrombin. Supernatants covering the FG layers were harvested from the gelled FG containing wells after 2, 24, 48 and 72 hours. F98 glioma cells in suspension were incubated with the photosensitizer aluminum phthalocyanine disulfonate (AlPcS2a, 1 μg/ mL) for 2 hours, washed and formed as 3D spheroids. The spheroids were incubated with BLM or DOX released from FG. One hour later, the spheroids were treated with light (PCI), λ = 670 nm, from a diode laser at an irradiance of 2.0 mW/ cm2. Spheroid growth was monitored for an additional 14 days. Results: At the BLM or DOX concentrations used in these experiments to load the FG, spheroid growth was not significantly influenced by the BLM or DOX that was released from the FG gels. In contrast, spheroid growth was significantly inhibited or completely suppressed by PCI of released drug from either FG-BLM or FG-DOX. Conclusion: The results of the present study show that drug was released in a non-degraded form for an extended time period. The growth inhibition caused by either FG released BLM or DOX was significantly enhanced by AlPcS2a mediated PCI.

Local Drug Delivery by Fibrin Glue for Glioma Treatment: Enhancing Drug Efficacy by Photochemical Internalization (PCI)