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Hancornia speciosa is a fruit tree that is in the process of domestication. This time the propagation in vitro when well developed can promote conditions for the production of quality seedlings for this fruit. In vitro culture technologies are of great importance for conservation programs of genetic resources and genetic improvement of mangabeira. The objective of this study is to establish a methodology the micropropagation of Hancornia speciosa , Gomes, of three varieties ( cuyabensis , gardneri and pubescens ) by in vitro germination of seeds under different growth regulators. Fruits were collected at School of Agronomy of the Federal University of Goias, transported to the Laboratory of Biotechnology, Federal Institute Campus Urutai - Goias, inoculated for germination in test tubes containing MS growth medium with 50% of salts concentrations, with five different concentrations of IBA. It was observed that the in vitro germination of cuyabensis , gardneri, and pubescens were 66.00 %, 64.05 % and 76 %, respectively. The beginning of germination occurred from three days for gardneri variety, six days for pubescens variety , and eigh day for cuyabensis variety, extending to the 16 days for gardneri variety, and 28 days for the other varieties. Explants of different varieties differ in their manifestations when cultured in vitro. The aseptic method was effective for the control of fungal agents

In vitro morphogenesis of different mangabeira (Hancornia speciosa gomes) varieties from the savanna of the Goiás