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The advent of sex-sorting-sperm sex selection of offspring became a great advantage, especially for dairy farms, which need new heifers for maintenance of his herds, and for beef cattle breeders, who crave more males for meat production. Currently, the only known technique capable of separating the populations of X and Y mammals sperm cells with efficiency and purity above 90% is flow cytometry. The best way of using sexed semen is in vitro fertilization, due to the small number of available sperm after sexing. Several factors can influence the results of in vitro embryos production using bovine sexed semen; among them there are the low rates of fertilization, cleavage, blastocyst, pregnancies, partially sperm capacitation, diluted semen samples and variation among bulls. Several studies have been conducted in order to assess the genetic and epigenetic alterations resulting from the use of sexed semen in the production of bovine embryos in vitro. Therefore, this paper aims to conduct a literature review on the limitations and advantages of using sexed semen, with emphasis on the technique of flow cytometry, during in vitro embryo production in cattle.

Produção de embriões bovinos in vitro com sêmen sexado