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The goals of this study were to determine biochemical biomarkers and histopathological changes in addition to exploring insulin as mono-therapy as well as insulin plus some medical plants include Garlic, Fenugreek (Trigonella foenum-graecum L.) and Black seeds (Nigella Sativa) as feed additives in dogs with experimentally induced diabetes mellitus (DM). A total of 15 clinically apparent healthy adult dogs were involved in this study and divided into group 1: included five normal adult dogs served as non-diabetic control group, group 2: Included five adult dogs that were subjected to experimentally diabetes mellitus by intravenous (IV) injection of alloxan and treated by with insulin only and group 3: Included five adult dogs that were subjected to experimentally diabetes mellitus by IV injection of alloxan and treated by with insulin plus once daily supplement with herbal therapy. In conclusions, the therapeutic effects of some medical plants include Garlic, Fenugreek (Trigonella foenum-graecum L.) and Black seeds (Nigella Sativa) increased the potency and glycemic control of insulin in animals with diabetes mellitus. Therefore, it is recommended adding the previously mentioned herbs as feed additives to improve the potency of insulin to control DM in dogs.

The effects of some medicinal plants with insulin on the inflammatory and metabolic responses in dogs with induced diabetes mellitus.