English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Tuberculosis is one of the major causes of mortality and morbidity in childhood. It is challenging to diagnose in childhood population due to difficulty in collection of sample and paucibacillary nature. Despite of considering mycobacterium culture as gold-standard test, it is time consuming (4-8 weeks) while traditional ZN stain is poorly sensitive or specific. In 2013, WHO endorsed Cartridge Based Nucleic Acid Amplification Test (CBNAAT) for diagnosis of TB. Still, there is lack of adequate research on TB detection efficacy of CBNAAT over ZN stain or culture. Aims & Objective: Comparison of CBNAAT over ZN stain & mycobacterium culture, in terms of Sensitivity, Specificity, Positive Predictive Value, Negative predictive value. Materials & Methods: Total of 565 patients admitted to Paediatric Medicine department over 2 years from July 2017 to July 2019 with clinical signs (radiological) and symptoms of local & disseminated tuberculosis and history of close contact were selected and grouped under pulmonary and extrapulmonary categories. Each sample was analyzed by CBNAAT, ZN smear and culture. Results: In the pulmonary group, CBNAAT detected 43 out of 185 sputum samples (23.2%), 110 out of 308 gastric lavage samples (35.7%), 0 out of 4 BAL samples. Besides, in extrapulmonary group6 out of 41 CSF samples, 3 out of 15 pleural fluid, 0 out of 7 ascitic fluid and 3 out of 5 LN biopsy samples were detected by CBNAAT. Sensitivity, specificity, positive predictive value, negative predictive value of CBNAAT in reference to ZN stain was 100%, 92.1%, 70.6% and 100% while in reference to culture was 93.2%, 99.1%, 96.2% and 97.2% respectively. Conclusion: CBNAAT assay is highly sensitive and specific to detect tuberculosis rapidly comparing to the traditional tests with rifampicin resistance associated with mutation of rpoB gene in a single test. It also helps in avoiding injudicious use of anti-tubercular drugs.

saudi journal of medical and pharmaceutical sciences

Is Cartridge Based Nucleic Acid Amplification Test (CBNAAT) Better Than Conventional Tests in Diagnosing Childhood Tuberculosis? Evidence from a Tertiary Care Hospital in Eastern India