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Original Research Article This study was aimed to investigate the immunostimulatory and hepatoprotective effects of different levels of herbs extracts like Withania somnifera, Liquorice,Alium sativum and Berberis lycium used to investigate the improvement in immunity and liver function at Agricultural University Peshawar, Pakistan. For this experiment two hundred and forty (240) chicks were randomly assigned in to four major groups A, B, C and D. Each group was further divided in to two sub-groups. Each subgroup was replicated three times carrying 10 chicks per replicate. One of the subgroups was vaccinated against ND (New castle disease), IB (Infectious bronchitis) and IBD (Infectious Bursal disease) according to schedule.Group A was kept as control, while B, C and D were treated with Withania somnifera , Licorice, Alium sativum and Berberis lycium extract at the rate of (5gms + 2.5gms + 2gms + 8gms), (10gms + 5gms + 3gms + 9gms), (15gms + 7.5gms + 4gms + 10gms) gm/lit of water respectively. AST (Aspartate amino transfarase) and ALT (Alanine amino transfarase)was significantly (P<0.05) reduced in group B than rest of the groups, while serum protein and alkaline Phosphatase was significantly (P<0.05) affected in group B as compared with other groups. Chicks were reared in cages in an open sided house. The data was recorded for immunostimulant and hepatoprotective effect. ND, IBD and IB was significant (P<0.05) higher in group B.It is concluded that Withania somnifera, Licorice, Alium sativum and Berberis lycium) extract @ of (10gms + 5gms + 3gms + 9gms) gm/L of water could be effectively utilized to improve immunity and liver function.

scholars journal of agriculture and veterinary sciences

Immune Stimulatory and Hepatoprotective Effects of Poly Herbs (Withania somnifera, Liquorice, Allium sativum and Berberis lycium) Mixture Extract in Broilers