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Background: 25-hydroxyvitamin D-1-α-hydroxylase is a key enzyme for conversion of 25-(OH)-D to 1,25-(OH)2-D and is extra renally produced in various tissues. The objective of the study was to determine the levels of enzyme 1-α-hydroxylase in response to supplementation with vitamin D in premenopausal women.Methods: This single-arm pre-post interventional study, included premenopausal females (n=82) and their 1-α hydroxylase levels were measured from peripheral blood. Vitamin D supplements were given and vitamin D levels and α-1 hydroxylase levels were measured through ELISA. The serum vitamin D levels below 30ng/ml (75nmol/L), were considered vitamin D deficient. The correlation between serum vitamin D levels and 1-α-OHase levels was determined using Spearman’s Rank Correlation test, Wessa P Spearman Rank Correlation in Free Statistics Software. Relationships between variables were done through paired t-test and p&lt;0.05 was considered significant.Results: The levels of 1-α (OH)ase varied in response to supplementation, with most values observed between 10-20ng/ml. The average increase in serum vitamin D and 1-α-(OH)ase levels was 9.49ng/mL and 4.66ng/mL respectively. In total 50(60.9%) samples revealed a decrease in 1-α (OH)ase levels, and 32(39.1%) samples increased. Data analysis showed t (84) = 1.214, p=0.228 for serum 1- α (OH)ase. For post-supplementation the results showed no association between (rs = 0.04982, p = 0.65671) the two variables.Conclusion: Serum levels of 1-α-hydroxylase in premenopausal Pakistani females indicated variation when vitamin D was supplemented, highlighting a non-linear relationship. This shows involvement of other unknown factors in Vitamin D metabolism, which requires to be investigated in future research.Keywords: Biopsy; Inflammation; Hydroxylase.

Assessment of 1 α-Hydroxylase in Vitamin D- Deficient Premenopausal Pakistani Females