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Nig. 1.Entomol. 33: 73-82 (2017) ABSTRACT: Laboratory bioassay were conducted to assess the insecticidal efficacy of raw diatomaceous earth (ROE), Jatropha curcas seed powders (.ISP) alone and in combination against Callosobruchus subinnotatus in stored bambara nut seeds. The study was carried out under ambient laboratory conditions at 30 ± 2 "C and 55 ± 4 % relative humidity using Completely Randomized Design. The seeds were admixed with the ROE, JSP each at tbrce dose rates as unitary (ROE: 0.5, J.O and l.5 g/I 00 g) and (JSP: 0.5, 1.0 and 2.0 gll 00 g) and their binary combination at (0.5+0.5, 1.0+ 1.0, 1.5+2.0 gl1 OOg).Actcllic' dust at the rate of2.0 g/kg and untreated control were used as positive and negative controls, respectively. Each glass jar (500 ml) was later infested with 30, 1-2-days old C. subinnotatus of mixed sexes. Adult mortality was assessed from 24 hours to 96 hours after treatment (HAT). FI, F2 adult emergence at 32 and 74 days after treatment (OAT) and percentage seed damage and weight loss were assessed after 90 days. The germination ofbambara nut seeds were evaluated at the beginning (before infestation) and after 90 days oftreatments and infestation. The results revealed that binary combination of test powders were most effective than either ROE or JSP as unitary applications. The highest dose rates 0 [the binary combination resulted in 100% adult mortality at 72 HAT. Similarly, all treatments demonstrated reduced mean number of F, F2adult emergence, seed damage and weight loss compared to the untreated control. Significantly, higher germination percentage was recorded in all treatments before infestation. However, after 90 days of treatments and infestation, the germination capacity of treated seeds decreased, which was significantly different from the untreated control. Bambara nut seeds treated with binary combination gave the best protection against C. subinnotatus and thus can be incorporated into integrated stored pest management.

nigerian journal of entomology

Insecticidal Efficacy of Raw Diatomaceous Earth, and Jatropha curcas Seed Powder Alone, and in Combination against Callosobruchus subinnotatus (Pic) [Coleptera: Chrysomelidae) in Stored Bambara Nut (Vigna subterranea L. Verdc.) Seeds