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2 Abstract: This work investigate the Phytochemical constituents of Pisoniaalba (PA) leaves using standard protocols and to determine its DNA damage inhibition and Antiurolithiatic capability. Dried leaves of Pisoniaalba were used to screen and quantify the phyto constituents in different organic solvents extracts that are analysed by GCMS, FTIR and HPTLC. In the presence of PUC18 plasmid DNA, the DNA damage inhibition assay was performed by photolysing H2O2 with UV radiation and agarose gel electrophoresis with irradiated DNA. The invivo antiurolithic assay was performed by nucleation method. Pisoniaalbain's phytochemical analysis reveals the existence of secondary metabolite components including glycosides, resins, phenols, terpenoids, flavonoids, tannins, steroids, and alkaloids, etc.. ELPA express the maximum quantity of phenols, flavonoids, alkaloids and terpenoids which were 30.44 ±0.65 mg TAE/g extract, 28.51 ± 1.19 mg RE/g extract, 28.08 ± 0.08 mg of AE/g of extract, 29.94 ± 0.32 mg RE/g extract respectively. The GC-MS assessment findings confirmed the presence of nine ELPA phyto compounds accompanied by eleven ALPA phytoc ompounds. It was also observed that Pisoniaalba leaves prevents DNA damage in UV and H2O2 treated plasmid DNA. The invitro Antiurolithiatic research indicates that ELPA was more efficient in blocking calcium oxalate nucleation. From the obtained results it was proven that the Pisonia alba leaves has potential secondary metabolites with therapeutic role mainly against urolithiasis. Both invitro and in vivo analysis demonstrates that ELPA significantly reduces crystal formation that are causative of renal stones. These evidences suggest that Pisoniaalbacan be further investigated for the prevention and treatment of urolithiais.

Screening of Phytochemical Compounds and Assessment of Antiurolithiatic Activity of Pisonia Alba Leaves Extracts against DNA Damage