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Green Ladybugs (Nezara viridula L.) are important pests for several food crops such as soybeans, rice and corn. One of the efforts to control this pest is the use of entomopathogens, such as viruses, bacteria and fungi. One of the entomopathogenic fungi that has the potential to be exploited is the fungus Beauveria bassiana. The fungi B. bassiana has various of hosts. There are no data on the pathogenicity of B. bassiana isolated from several host species against green ladybugs (Nviridula), for that reason this study was conducted with the aim of knowing the pathogenicity of B. bassiana isolated from different hosts against green ladybugs (N. viridula).. This research was conducted at Laboratory of Biological Control Agents, the Center for the Protection and Quality Testing of Food Crops and Horticulture, the Regional Agriculture and Livestock Service Office of Siahaan, et al.,: Patogenisitas Beauveria bassiana (Bals.) Viull. yang  27 North Sulawesi Province. The research was conducted in-vitro using a completely randomized design (CRD) with 6 treatments, namely (Negative control, N1: suspension B. bassiana, WBC isolate (brown planthopper), N2: B. bassiana suspension, Jati Sari isolate as a positive control (Ministry of Agriculture Recommendation), N3 : B. bassiana suspension isolate Paraeucosmetus pallicornis (black ladybug), N4: suspension B. bassiana isolate Leptocorisa acuta (Rice ear bug) and N5: suspension B. bassiana isolate Scotinopara coarctata (rice black bug) Treatment using conidia concentration or density 10 / ml water, with 5 replications. Data were analyzed with Anava test 95% and probit analysis to determine LT50 (Lethal Time 50). The results showed that the fungus B. bassiana Jati Sari isolate had the highest pathogenicity, because this isolate was able to kill green ladybugs (N viridula) to 100% on the 5th day of DAT (the day after treatment), while the other isolates were all on the 6th DAT. While the value of LT50 (time needed to kill 50% of the insect test) was found in P. pallicornis isolates which were 2,1 days of DAT. Because LT50 in all isolate treatments was between the 2nd and 3rd day of DAT, the Anava test for mortality was carried out on the 3rd day of DAT. The highest percentage of mortality on the 3rd day of DAT was found in the treatment of P. pallicornis isolates with an average mortality of 78% followed by L. acuta and S. coarctata isolates each at 68% and Jati sari isolates at 58%, while the lowest mortality was found in the treatment of WBC isolates, namely 32%. There was no significant difference in pathogenicity between Jati Sari, P. pallicornis, L. acuta and S. coarctata isolates except for WBC isolates.

jurnal ilmiah sains/jurnal ilmiah sains

Patogenisitas Beauveria bassiana (Bals.) Viull. yang Diisolasi dari Beberapa Jenis Inang Terhadap Kepik Hijau, Nezara viridula L. (Hemiptera: Pentatomidae)