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In this research, Trichoderma spp. isolates collected from tomato fields in the “Cinturón Hortícola Platense” were evaluated in vitro against of Clavibacter michiganensis subsp. michiganensis, as well as its effect to prevent the bacterial canker in glasshouse conditions. Nine strains of Trichoderma spp. were obtained from soil samples using the serial dilution method. Its ability to suppress and inhibit pathogen growth was evaluated in an in vitro assay. All Trichoderma isolates showed ability to suppress the bacterial growth between 14 and 16%, just isolate A4 was capable to inhibit the pathogen development in 15.76%, approximately. Under glasshouse conditions, five Trichoderma spp. isolates were inoculated in 15 days seedlings using two methods: (1) soil inoculation during the transplant and (2) leaf inoculations three days before pathogen inoculation. Ten days after the transplant, the pathogen was inoculated by spraying a bacterial suspension with 1 x 10 CFU/ml, approximately. Five weeks after the transplant plant height, number of flowers and fruits were evaluated; results showed there were statistical differences between biocontrol treatments and the control. Tomato seedlings with leaves treated had bigger averages than those with soil inoculation. After eight weeks from transplant, disease symptomatology was evaluated. Approximately, 50% of pathogen control plants showed symptoms associated with the pathogen under glasshouse conditions, such us: marginal necrosis of leaflets and generalized slight wilting; the biocontrol strains had a positive effect over treated seedlings which were healthier than control ones, as well as they had better vegetative development and dry mass accumulation.

Selección y efectividad del uso de aislamientos de &lt;i&gt;Trichoderma&lt;/i&gt; spp. para el control del Cancro Bacteriano (&lt;i&gt;Clavibacter michiganensis&lt;/i&gt; subsp. &lt;i&gt;michiganensis&lt;/i&gt;) del tomate (&lt;i&gt;Lycopersicum esculentum&lt;/i&gt; Mill.)