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The reductive amination chemistry is a synthetic approach normally used in the development of conjugate vaccines. In this approach, a high carrier protein reactivity is desirable. This improvement occurs by converting the side carboxylate groups of aspartic and glutamic residues into their respective hydrazides. The measurement of this conversion is an analytical challenge due to sample complexity and low concentrations of analytes. The determination of relative activation rate will allow us to monitorize the reproducibility of the method bringing innovation to process quality control

Quality control process through monitoring hydrazide-activated protein reactions by LC-SRM/MS