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The incidence of tetanus is still a public health problem in Brazil. The biological product adopted by National Immunisations Programme (NIP) for prophylactic and therapeutic purposes is the Tetanus Antitoxin for Human Use (TAHU). The National Institute for Quality Control in Health (INCQS) is the Brazilian laboratory responsible for evaluating the quality of TAHU lots. Potency assays for TAHU employ a large number of mice by tetanus toxin neutralisation testing (TNT). The Toxin Binding Inhibition (ToBI) test is a promising alternative to replace the use of animals in testing.

Assessment of parameters for lot release of Tetanus Antitoxin for Human Use through potency assays by ToBI test