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METHODOLOGY Sectional study conducted in healthy units where syphilis diagnosis is usually made. The study population was composed by adults stratified in three groups: infected with HIV (N= 174), pregnant women (N=170) and neither pregnant nor HIV infected (N=149). The syphilis status wasn ́t known before study enrollment. Whole blood, serum and plasma specimens were used for study objective. Reference tests for treponemal and nontreponemal antibody tests were TPHA and VDRL, respectively, following the flowchart of testing from Brazilian Ministry of Health. The sensitivity, specificity, positive and negative predictive values (PPV/NPV) has been calculated separately for each study group and biological specimen. The main outcome was the combined results of nontreponemal and treponemal DPP assays compared with TPHA positive combined with a VDRL with titer of 1:8.

Accuracy of a DPP Syphilis Bio-Manguinhos assay for simultaneous detection of nontreponemal and treponemal antibodies in patients with acquired syphilis