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Production of monoclonal antibodies involves in vivo or in vitro procedures. In vivo production presents great variability and difficulty of scaling-up, as well as ethical problems. In vitro processes are robust and achieve high cell densities, an easy scaling-up, and a better control/regulation of the process. The most widely used in vitro systems for antibody production include spinners and rollers flasks and bioreactors. Culture conditions have a significant impact on the cell growth and antibody production. In this work, D1-4G2-4-15 hybridoma, producer of monoclonal antibodies anti-flavivirus group antigen, were cultivated. Cellular viability, cell growth rate, IgG production, glucose and L-lactate concentrations have been used as investigation criteria in order to establish the optimized conditions of antibody production in spinner flasks.

Production of monoclonal antibodies in suspension culture of D1-4G2-4-15 hybridoma using spinner flasks system