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D1-4G2-4-15 hybridoma is responsible for secreting a monoclonal antibody (mAb) against Flavivirus group antigen and is currently used in different projects of vaccine development at BioManguinhos. Despite considerable progress in cell culture techniques, fetal bovine serum (FBS) supplemented media are still commonly used during hybridoma growth and maintenance in vitro. However, its use is controversial due to batch-to-batch composition variations, increased safety risk, low mAb yields after purification steps and ethical aspects related to the use of animals. In this context, adaptation of the hybridoma culture to serum-free medium meets regulatory concerns regarding the safety of animal-derived products.

Optimized production of monoclonal antibody used in Flavivirus immunoassays for different projects of vaccine development