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Methods: A precore fragment with 109 bp was cloned and serial diluted to standard curve construction. The calibration of the HBV DNA values was performed against OptiQuant® HBV-DNA Quantification Panel, Acrometrix Europe B.V.). Specifically, serial dilutions of the standard ranging from 2x10 to 2x10 were tested. Based on a linear regression, a conversion formula was calculated for the in -house measurements (copies/mL) to the international standard units (IU/mL). The correlation between Acrometrix kit and in house assay was performed by Pearson ́s test, using GraphPad 5.0 to fit regression lines between IU/mL and copies/mL.

Development of a sensitivity and cost-effective real time pcr: measuring a wide range of HBV DNA concentrations