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We induced middle cerebral artery occlusion (MCAO) in rats using silicone-coated vascular embolus. We transplanted mouse embryonic stem cells after MCAO. Rats were tested behaviorally using motor and sensory function with neurological assessment. Functional effectiveness of the mES transplanted was gradually improved the function of sensory neuron and motor neuron. This study demonstrated that the transplanted cells have synaptic connection in the recipient brain. We suggested that stem cell transplantation can have a positive effect on behavioral recovery and reduction of infarct size in focal ischemic rats. Therefore, we concluded that mES cells may have useful tool for treatment in neurological diseases.

Localization of Ischemic Brain Region After Mouse Embryonic Stem Cell Transplantation