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Aflatoxigenic fungi are most common filamentous fungi that synthesis aflatoxins and represent the major fungalpathogens to agricultural products. Aflatoxins remain a major threat to global food security, these molecules could beresisted into food during processing and in additional may remain within the food chain. Aflatoxins are carcinogenic,hepatotoxic, mutagenic, teratogenic, can inhibit numerous metabolic systems and immunosuppressive properties.Studies of aflatoxigenic strains can help to enhance strategies control and prevent aflatoxigenic fungi contaminationand aflatoxins production in foodstuffs. In this study, isolation of Aspergillus species was based on morphologicalcharacteristics including the mycelium growth pattern, color, and properties of fruiting bodies of the fungi. Theinnovated technique loop-mediated isothermal amplification assay was applied to amplify Norsolorinic Acid gene.The loop-mediated isothermal amplification have been optimized by combination of the rapidity, simplicity andspecificity to detect the target genomic DNA in the reactions. The amplification curves monitored by Loopamp realtimeTurbidimeter were analyzed in order to distinguish aflatoxigenic and non-aflatoxigenic strains.Overall, the results showed that the loop-mediated isothermal amplification method was effective in detectingaflatoxigenic strains with high specificity of 71.5% and sensitivity under lower concentrations of DNA. In additional,it was faster than the conventional polymerase chain reaction. The loop-mediated isothermal amplification assaydescribed in this study might be a promising tool for prediction potential threats by aflatoxigenic fungi and aflatoxinsrisk in food and commodities.

journal of plant pathology and microbiology

Application and Evaluation of the Loop-Mediated Isothermal Amplification assay for the Detection of Aflatoxigenic Fungi Contaminants of Rice Grains in Kenya