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Purpose:  This paper established the application of synthesized graphitic carbon nitride nanosheets (GCNNs) as an influential dispersive solid phase extraction (DSPE) adsorbent in extracting methamphetamine from complicated urine media coupled with high performance liquid chromatography.  Methods:  The graphitic carbon nitride nanosheets (GCNNs) was synthesized easily and applied as adsorbent in the extraction process. The effective extraction parameters were investigated by one-parameter-at-a-time. Under optimized conditions the method was validated.  Results:  The calibration curve was plotted in the concentration range of 50-1500 ng/mL through the optimized conditions and the proposed method was validated. The method was used for the analysis of positive urine samples and showed satisfactory results with the average 99.7% relative recovery.  Conclusion:  The results persuade the capability of this novel method in analyzing of the positive urine samples in diverse clinical and forensic laboratories.

advanced pharmaceutical bulletin

Application of Carbonic Nanosheets Based on Urea Precursors as Dispersive Solid Phase Extraction Adsorbent for Extraction of Methamphetamine from Urine Samples