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Purpose:  Estriol (ERL) is a type of hormone among the groups of estrogen hormone that was detected through the voltammetric technique by constructing an electrochemical sensor based on the octoxynol-9 modified graphite paste electrode (OXL-9MGPE).  Methods:  Using the strategy of cyclic voltammetry (CV) and differential pulse voltammetry (DPV) with a bare graphite paste electrode (BGPE) immobilized with OXL-9, ERL electro-oxidation has been assessed in 0.2 M phosphate buffer solution (PBS) of pH 6.0. The fabricated electrode has substantial electrochemical sensing efficiency, and the ERL oxidation at the OXL-9MGPE was the irreversible process. The surface morphological characteristics of BGPE and OXL-9MGPE were differentiated with the help of field emission scanning electron microscopy (FE-SEM).  Results:  The impact of various factors such as scan rate, pH, reproducibility, repeatability, and stability on the electro-oxidation of ERL was evaluated. Techniques of CV and DPV were utilized to determine ERL, dopamine (DAN), and uric acid (URA) simultaneously with the projected sensor. The peak current was varied with ERL concentration in the range from 4×10 -5 to 1.2×10 -4 M at OXL-9MGPE. From this, the detection limit 1.4×10 -6 M and limit of quantification (LOQ) 4.7×10 -6 M have been attained.  Conclusion:  As a result, OXL-9MGPE was successfully achieved as an electrochemical detector for the electro analysis of ERL via the CV technique.

advanced pharmaceutical bulletin

Surfactant Modified Graphite Paste Electrode as an Electrochemical Sensor for the Enhanced Voltammetric Detection of Estriol with Dopamine and Uric acid