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Background: Accuracy of measurements obtained from cone-beam computed tomography (CBCT) images, which is a diagnostic tool in dentistry, is an important issue. The aim of this study was to compare the ability of different operators in measuring dimensions using CBCT software. Methods: In this experimental study, 35 different areas using opaque objects and drilling cavities were prepared on 3 phantoms which were made from fresh beef ribs. Then each phantom was scanned by CBCT Promax 3D. The mentioned areas were measured on CBCT images 2 times with one week interval by four observer groups consisting two radiologists, two periodontists, two maxillofacial surgeons, and two general dentists. Obtained measurements from each group of observers were compared with those of other groups and also with measurements of a digital caliper as a gold standard by intra-class correlation coefficient (ICC). Then the measured dimensions, with respect to their application, were divided into three clusters including cluster 1: 2-7 mm, cluster 2: 7-16 mm, and cluster 3: more than 16 mm. T test was used to compare the mean value of each cluster with the mean value of the gold standard. Results: In general, based on ICC, interand intra-observer agreement, agreement between observer groups, and agreement between each group and the gold standard were significant. The results of t test showed a significant difference between the mean value of data and that of gold standard in clusters 1 and 3. Conclusions: Generally, high accuracy and reliability were reported for different specialists of dentistry and general dentists in measuring the dimensions of objects and cavities in CBCT images. *Correspondence to Amir Eskandarloo, Department of Oral Radiology, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran. Tel:+98-81-3838105, Email: dr.eskandarloo@ gmail.com Background There are various indications for the use of imaging in the field of dentistry and oral surgery. Radiographic examination is regarded as one of the important diagnostic tools in dental treatments (1). Cone-beam computed tomography (CBCT) was an innovation in maxillofacial imaging. It can produce a rapid volumetric image with a low-dose scan of the patient (low mA). CBCT provides multiple planar images of both jaws by a single rotational scan (2-5). This imaging system can provide 3-dimensional visualization of all tooth surfaces as digital images (6). CBCT is a user friendly and autorun software package. Many clinicians use the capability of linear measurement in measuring the distances between anatomic landmarks or measuring bone thickness for treatment planning and surgery. Therefore, any error could affect treatment processes (7-10). The reliability of radiographic angles, ratio, and length measurements are the key factors that contribute to the clinical decisions in treatment plans (11). CBCT can be used as a diagnostic tool that produces The Ability of Different Observers in Measuring Dimensions of Objects and Cavities in Cone Beam-CT Images Received Dec. 2 2019 Accepted Dec. 15 2019 ePublished Dec. 3