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A mouse monoclonal antibody (mAb), designated mNI-58, was produced. The mNI-58 markedly induced spread formation of the phorbol myristate acetate (PMA)-stimulated U937 cells (referred to as PMA-U937 cells) but not that of the resting U937 cells. This spread formation was completely blocked in the presence of cytochalasin D or cycloheximide. It was partially blocked by an mAb against CD18 (lymphocyte function-associated antigen-1 beta). These results suggest that mNI-58 is capable of transducing a signal for spread formation of the PMA-U937 cells.

Monoclonal Antibody mNI-58 Inducing Morphological Changes in a Human Monocyte-Like Cell Line, U937, Stimulated with Phorbol Myristate Acetate(PMA).