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A mouse monoclonal antibody (mAb), designated as mNI-11, has been produced. This mAb strongly induced homotypic cell aggregation (HCA) of monocyte-like cell line U937 cultured with conditioned medium derived from PHA-stimulated peripheral blood mononuclear cells and cytochalasin B. HCA occurred at an optimal temperature of 37 C but not 4 C. mAbs to CD18 (lymphocyte function-associated antigen-1 beta; LAF-1 beta) and CD54 (intercellular adhesion molecule-1; ICAM-1) completely inhibited this HCA. These results suggest that mNI-11 strongly induces HCA of the U937 cells in an activated form after stimulation with conditioned medium and cytochalasin B.

nihon saikingaku zasshi

Monoclonal antibody mNI-11 strongly induces homotypic cell aggregation of monocyte-like cell line U937: effects of conditioned medium and cytochalasin B.