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The experiment was carried out with the objective of evaluating the antioxidant effects of the addition of eugenol in the TRIS-yolk extender in cryopreservation of bovine sperm. Twenty ejaculates from four Curraleiro Pé-Duro cattle were used, after the collection the semen was diluted in TRIS-Gem and divided into three aliquots; control, treatment in concentrations of 10μM and 50μM of eugenol. Subsequently the samples were filled in 0.25ml packaged in straws and cryopreserved. Then the straws were kept in equilibrium for 60 minutes at 5oC, to then be cryopreserved in a standard cryopreservation protocol using the TK 3000® machine, and finally the packaged in straws were dipped in liquid nitrogen (-196o C) and stored in a cylinder cryogenic. After 30 days, post-cryopreservation analyzes were performed. Sperm evaluations included sperm motility and vigor (TTR), at 0, 60, 120 and 180 minutes, determination of plasma and acrosomal membrane integrity, sperm membrane functionality (HOST), sperm kinetics, sperm lipoperoxidation quantification, quantification GSH and in vitro fertilization test. It was observed that eugenol supplementation in concentrations of 50μM differed significantly (P <0.05) from the other treatments, inducing an increase in the quantification of GSH, a reduction in the determination of MDA, an increase in VSL and preserved the integrity of the membranes plasma and acrosomal. In conclusion, the use of eugenol in the cryopreservation of semen in bulls is suggested, in order to limit the damage induced by cold. Brazilian Journal of Development Braz. J. of Develop., Curitiba, v. 6, n. 11, p. 86336-86355, nov. 2020. ISSN 2525-8761 86338

O USO DO EUGENOL NA CRIOPRESERVAÇÃO DO SÊMEN BOVINO / THE USE OF EUGENOL IN THE CRYOPRESERVATION OF BOVINE SÊMEN