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The isomers of ‰uoro-butyrylfentanyl, ‰uoro-isobutyrylfentanyl, and ‰uoromethoxyacetylfentanyl, in which the position of ‰uorine on the Nphenyl ring varies, were synthesized, characterized, and diŠerentiated by infrared (IR) spectroscopy, liquid chromatography/mass spectrometry (LC/MS), and gas chromatography/mass spectrometry (GC/MS). The isomers could be clearly diŠerentiated by their IR spectra. In the LC/MS chromatograms, the separation of the ‰uoro-butyrylfentanyl and ‰uoro-isobutyrylfentanyl isomers was insuŠicient. However, in the GC/MS extracted ion chromatograms, all compounds were completely separated. The LC/MS and GC/MS mass spectra of the isomers were similar, demonstrating that it is diŠicult to distinguish the positional isomers of ‰uorinated fentanyl analogs by their mass spectra.

Characterization and differentiation of positional isomers of fluoro-fentanyl analogs by a combination of instrumental analyses