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The present study is carried out with the lactic acid bacteria isolation and identification such as Lactobacillus fermentum from curd sample. Five isolates of Lactobacilli supernatant is examined for acid and antibacterial activity production. The extracted compound showed antibacterial activity against the microorganisms. Lactobacillus fermentum showed better antimicrobial activity. Antibacterial activities LAB are dietary supplements of live microorganisms thought to be healthy for the host organisms. In this study, lactic acid bacteria are isolated from 2 different curd samples. A total of 20 strains are studied for their characterization, from that 2 potential strains Lactobacillus acidophilus and Lactobacillus plantarum isolated from curd. The potential strains are studied by curd production, antibacterial and bactericidal activity also their antibiotic susceptibility. The quality of curd is analyzed by qualitatively and quantitatively by nutrient evaluation. The antibiotic resistance of potential strain is studied using the good resistance activity to the antibiotics. The curd produced by those strains has good quality and quantity of nutrients.

To Study Characterization and Antibacterial Activity of Bacteriocin Producing Lactobacillus Isolated from Curd