English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The invasive bivalves Corbicula spp. and Limnoperna fortunei predominate in South American rivers. They can be sympatric in distribution, and because their larval stages are morphologically similar, monitoring them in zooplankton using microscopy protocols is often inefficient, producing ambiguous results. We designed a pair of primers to amplify a fragment of the mtDNA cytochrome c oxidase subunit I of Corbicula species. A multiplex reaction, containing the specific primer pair and a pair of universal primers (to control for the quality of the DNA in the sample) was tested with regards to specificity and ability to detect Corbicula spp. larvae in plankton samples that also contain other species in different proportions. Our molecular protocol allows for fast and accurate detection of Corbicula spp. even when concentrations of these species are low in samples, which is useful when examining large volumes of ballast/piped water. Further, the protocol is valuable for the monitoring/prospecting of early stages of the life cycle of Corbicula spp. in watersheds that have been invaded, or which are considered at risk of invasion by these species.

Looking for a needle in a haystack: molecular detection of larvae of invasive Corbicula clams