English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Blooms of cyanobacteria are frequent in Brazilian water reservoirs used for drinking water. The warning for the presence of potential toxin-producing cyanobacteria is typically based on time-consuming microscopy, rather than specific molecular detection of toxic genes in cyanobacteria. In this study, we developed a quantitative PCR assay for the detection of cyanobacteria producing the neurotoxin saxitoxin (STX). The assay targets the sxtA gene in the sxt gene cluster. Potential and dominant STX-producers in the Itupararanga reservoir were the genera Raphidiopsis, Aphanizomenon and Geitlerinema. Numbers of the sxtA gene varied from 6.76 × 103 to 7.33 × 105 cells mL−1 and correlated positively with SXT concentrations in the water. Concentrations of STX and the sxtA gene also correlated positively with TN:TP ratio and pH, but correlated negatively with inorganic nutrients and turbidity, confirming that regulation of the SXT production was impacted by environmental variables. In contrast, the occurrence of another cyanotoxin, microcystin, did not correlate with any environmental variables. The developed qPCR assay was found to be a rapid and robust approach for the specific quantification of potential STX-producing cyanobacteria and should be considered in future investigations on toxic cyanobacteria to provide an early warning of potential toxin episodes.

water

Influence of Environmental Factors on Occurrence of Cyanobacteria and Abundance of Saxitoxin-Producing Cyanobacteria in a Subtropical Drinking Water Reservoir in Brazil