English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

A complex of begomoviruses ( Geminiviridae ) can cause severe tomato yield losses in the neotropics. Here, next-generation sequencing was employed for large-scale assessment of single-stranded (ss)DNA virus diversity in tomatoes either harboring or lacking the large-spectrum begomovirus tolerance Ty -1 gene. Individual leaf samples exhibiting begomovirus-like symptoms ( n = 107) were field-collected, circular DNA-enriched, subdivided into pools (with and without Ty -1), and Illumina-sequenced. Virus-specific PCR and Sanger dideoxy sequencing validations confirmed 15 distinct ssDNA virus/subviral agents (occurring mainly in mixed infections), which highlight the potential drawbacks of employing virus-specific resistance in tomato breeding. More viruses (14 versus 6 species) were observed in tomatoes without the Ty -1 gene. A gemycircularvirus ( Genomoviridae ), a new alpha-satellite, and two novel Begomovirus species were identified exclusively in samples without the Ty -1 gene. A novel begomovirus was found only in the Ty -1 pool, being the only species associated with severe symptoms in Ty -1 plants in our survey. Our work is the first step towards the elucidation of the potential begomovirus adaptation to Ty -1 and its specific filtering effects on a subset of ssDNA viral/subviral agents.

Metagenomics of Neotropical Single-Stranded DNA Viruses in Tomato Cultivars with and without the Ty-1 Gene