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Simultaneous determination of twenty-seven mycotoxins in ready-to-eat food samples using &amp;ldquo;Quick Easy Cheap Rough and Safe&amp;rdquo; (QuEChERS) extraction and chromatographic methods coupled to mass spectrometry in tandem is described in this study. Mycotoxins included in this survey were aflatoxins (B₁, B₂, G₁, G₂), enniatins (A, A₁, B, B₁), beauvericin (BEA), fumonisins (FB₁, FB₂), sterigmatocystin (STG), deoxynivalenol (DON), 3-acetyl-deoxynivalenol (3-ADON), 15-acetyl-deoxynivalenol (15-ADON), nivalenol (NIV), neosolaniol (NEO), diacetoxyscirpenol (DAS), fusarenon-X (FUS-X), zearalenone (ZEA), &amp;alpha;-zearalanol (&amp;alpha;ZAL), &amp;beta;-zearalenone (&amp;beta;ZAL), &amp;alpha;-zearalenol (&amp;alpha;ZOL), &amp;beta;-zearalenol (&amp;beta;zol), T2, and HT-2 toxin. The method showed satisfactory extraction results with recoveries ranging from 63 to 119% for the different food matrix samples. Limits of detection (LOD S ) and quantification (LOQs) were between 0.15⁻1.5 &amp;micro;g/kg and 0.5⁻5 &amp;micro;g/kg, respectively. The method was successfully applied to the analysis of 25 ready-to-eat food samples. Results showed presence of deoxynivalenol at 36% of samples (2.61⁻21.59 &amp;micro;g/kg), enniatin B at 20% of samples (9.83⁻86.32 &amp;micro;g/kg), HT-2 toxin at 16% of samples (9.06⁻34.43 &amp;micro;g/kg), and aflatoxin G₂ at 4% of samples (2.84 &amp;micro;g/kg). Mycotoxins were detected mainly in ready-to-eat food samples prepared with cereals, vegetables, and legumes, even at levels below those often obtained from raw food.

Evaluation of Mycotoxin Residues on Ready-to-Eat Food by Chromatographic Methods Coupled to Mass Spectrometry in Tandem