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Critical infrastructure is a complex system whose disruption or failure results in significant impacts on state interests, i.e., territorial security, economy, and the basic needs of the population. The current European Critical Infrastructure Protection Model does not allow the direct identification of critical elements at the regional level. Based on this, the paper brings a proposal for a unified system of critical infrastructure design based on a bottom-up approach. It is a progressive approach, utilizing contemporary trends in the application of science-based knowledge to critical infrastructure. A holistic view of this issue allows us to take into account the needs and preferences of the population, the preferences of the stakeholders and the local conditions of the region under consideration. The novelty of this approach is seen, in particular, in the identification of regional critical infrastructure elements through an integral assessment of these elements’ failure impact, not only on the dependent subsectors, but also on the population (population equivalent) in the assessed region. The final part of the paper presents a case study demonstrating the practical application of the proposed system to the road infrastructure in the Pardubice Region of the Czech Republic.

Designating Regional Elements System in a Critical Infrastructure System in the Context of the Czech Republic